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Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Antibody Detection Kit is used to detect PRRSV-specific antibodies in pig serum, and to evaluate the serological auxiliary diagnosis of PRRS vaccine immunity or infection in pig farms. This kit has good detection sensitivity for the antibodies produced by the classic strains of PRRS virus and highly pathogenic strains.
This kit adopts the indirect ELISA method, and the purified PRRSV antigen is pre-coated on the microwell of the enzyme label strip. In the test, the diluted serum to be tested is added, and after incubation, if the sample contains PRRSV-specific antibodies, it will bind to the PRRSV antigen on the coated plate, and after washing to remove unbound antibodies and other components; Add the enzyme-labeled secondary antibody to specifically bind to the antigen-antibody complex on the detection plate; then wash to remove unbound enzyme markers; add TMB substrate solution to the microwell, form a blue product by enzyme catalysis, add After the reaction was terminated by the stop solution, the absorbance OD value in the reaction well was measured with a microplate reader at a wavelength of 450 nm.