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The African Swine fever (ASF) antibody detection kit is used to detect the African swine fever antibody in pig serum.
This kit adopts the indirect ELISA method, and the purified ASF antigen is pre-coated on the microwell of the enzyme label strip. In the test, add the diluted serum to be tested, and after incubation, if the sample contains ASF virus-specific antibodies, it will bind to the antigen on the coated plate, and after washing to remove unbound antibodies and other components; Add the enzyme-labeled secondary antibody to specifically bind to the antigen-antibody complex on the detection plate; then wash to remove unbound enzyme markers; add TMB substrate solution to the microwell, form a blue product by enzyme catalysis, add After the reaction was terminated by the stop solution, the absorbance OD value in the reaction well was measured with a microplate reader at a wavelength of 450 nm.